This study mainly focus on the potential antibacterial properties of various extracts (petroleum ether, chloroform, ethanol, and Aqueous) of aerial part of Andrographis paniculata, against nine human pathogenic micro-organism (Gram +ive: B. pumillus, B. subtilis, S. aureus, M. luteus, Gram –ive: E. coli, K. pneumoniae, P. aeruginosa, S. typhi, and S. abony) by using disk diffusion method at two concentrations 500 µg/ml, 1000 µg/ml. The MIC and MBC methods were also used. The extracts showed significant antibacterial activities against both gram (+ve) and gram (-ve) bacterial strains tested. Highest significant antibacterial activity was exerted by the aqueous extract against B. subtilis at 1000µg/ml (23.4mm) and the least activity by petroleum ether extract against P. aeruginosa at 500µg/ml (6.0mm). Aqueous extract of A. paniculata showed high antibacterial activity against B. pumillus, B. subtilis, S. aureus and M. luteus, whereas ethanol, chloroform and petroleum ether extracts showed intermediate effect against gram (+ve) and moderate against gram (-ve) bacteria. The inhibitory activities of all the extracts reported were compared with reference standard Ciprofloxacin (50µg/ml). The MIC range is in between (70-350µg/ml) depending on micro organisms and various extracts. An MIC of 70µg/ml shown in aqueous extract (1000µg/ml) against K. pneumoniae and S. typhi (75µg/ml) was considered to be best. The range of MIC value of petroleum ether extract (1000µg/ml) was (250-310µg/ml), chloroform extract (200-300µg/ml), ethanol (150-350µg/ml) and aqueous extract (70-160µg/ml). The MBC value ranged between (130-690µg/ml) depending on micro organism and various extracts. A potent antibacterial and hepatoprotective drug could probably be formulated from the plant extract of A. paniculata to cure the bacterial and heptotoxic infections. Our results confer the utility of this plant extracts in developing a normal broad spectrum antimicrobial agent.
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